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Serial Anrikning av Spermatogonial Stem og stamceller (SSCS) i Kultur for Utledning av langsiktige Voksen Mus SSC Lines
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JoVE Journal Biology
Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines

Serial Anrikning av Spermatogonial Stem og stamceller (SSCS) i Kultur for Utledning av langsiktige Voksen Mus SSC Lines

DOI:
10.3791/50017-v

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12:26 min

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February 25, 2013

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,
1Department of Surgery,Weill Cornell Medical College

Chapters

  • 00:05Title
  • 01:41Preparation of Feeder Cells
  • 03:54Dissociation of Mouse Testis Tissue to Obtain Donor Germ Cells
  • 06:17Plating Testicular Suspension and Colony Picking for First Passage
  • 09:00Subsequent Expansion and Passaging of SSCs by Trituration
  • 10:13Representative SSC Culturing Results
  • 11:51Conclusion

Summary

Automatic Translation

Automatic Translation

En enkel metode for å utlede og vedlikeholde spermatogonial stilk og progenitor cellelinjer fra voksne mus er presentert her. Metoden utnytter mater celler stammer fra det somatiske celle kupé av de voksne mus testis. Denne teknikken gjelder vanlige musestammer, inkludert transgene, knock-out, og knock-i mus.

Tags

Keywords: Spermatogonial Stem CellsSSCsAdult MouseIn Vitro CultureSerial EnrichmentProgenitor CellsTestisSelf-renewalDifferentiationNeonatalAdultSomatic CellsStem Cell NicheJK1 Feeder CellsPassaging

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