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Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
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Bioengineering
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JoVE Journal Bioengineering
Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

DOI:
10.3791/50059-v

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11:30 min

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December 06, 2012

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, ,
1McGowan Institute for Regenerative Medicine, 2Department of Bioengineering,University of Pittsburgh, 3Department of Cardiothoracic Surgery,Children’s Hospital of Pittsburgh of UPMC, 4Department of Surgery,University of Pittsburgh

Chapters

  • 00:05Title
  • 01:25Tissue Preparation and Experiment Setup
  • 04:41Tissue Rinses
  • 05:55Decellularization and Solution Perfusion
  • 08:27Disinfection and Final Processing
  • 10:29Decellularization of Porcine Hearts
  • 11:14Conclusion

Summary

Automatic Translation

Automatic Translation

A method to rapidly and completely remove cellular components from an intact porcine heart through retrograde perfusion is described. This method yields a site specific cardiac extracellular matrix scaffold which has the potential for use in multiple clinical applications.

Tags

Keywords: DecellularizationPorcine HeartRetrograde Coronary PerfusionExtracellular Matrix ScaffoldEnzymeDetergentAcidHypertonicHypotonicTrypsinTriton X-100Sodium DeoxycholatePerfusion Speed

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