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结合免疫荧光和三维保存的间期核荧光原位杂交DNA研究在3D核组织的变化
JoVE Journal
Biology
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JoVE Journal Biology
Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
DOI:

13:55 min

February 03, 2013

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Chapters

  • 00:05Title
  • 01:27DNA Probe Labeling with Fluorophores: Nick Translation
  • 03:37Probe Precipitation/Denaturation/Pre-annealing
  • 05:133-dimensional DNA FISH
  • 09:253-dimensional DNA FISH / Immuno-FISH
  • 10:40Combined Immunofluorescence and DNA FISH: Representative Results
  • 12:56Conclusion

Summary

Automatic Translation

在这里,我们描述了一个协议,用于同时检测组蛋白修饰免疫荧光法和DNA序列的DNA荧光原位杂交,然后通过的3D显微镜和分析(3D的免疫-DNA FISH)。

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