Journal
/
Biology
/
Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions

Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions

DOI:
10.3791/50102-v

•

15:33 min

•

August 04, 2013

•

, , , ,
1Departamento de Biología Molecular,Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), 2Servicio de Citometría de Flujo y Clasificación Celular (SECIF),Instituto de Investigaciones Biológicas Clemente Estable, 3Sección Bioquímica, Facultad de Ciencias,Universidad de la República

Chapters

  • 00:00Title
  • 02:02Preparation of the Cellular Suspension
  • 04:55Flow Cytometric Analysis
  • 08:29Results
  • 14:22Conclusions

Summary

Automatic Translation

Automatic Translation

A novel protocol for the mechanical preparation of testicular cell suspensions from rodent material, avoiding enzymes and detergents, is described. The method is very simple, fast, reproducible, and renders good quality cell suspensions, which are suitable for flow sorting and RNA extraction.

Tags

Testicular Cell SuspensionMammalian SpermatogenesisCell EnrichmentCell PurificationMechanical DisaggregationFlow CytometryGene Expression Studies

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

Generation of Single-Cell Suspensions from Mouse Neural Tissue

Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparation of Quality Inositol Pyrophosphates

Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Read Article