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米色/布里特细胞的间质血管细胞的分离和分化
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JoVE Journal Biology
Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
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米色/布里特细胞的间质血管细胞的分离和分化

DOI:
10.3791/50191-v

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07:22 min

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March 28, 2013

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, ,
1UCSF Diabetes Center and Department of Cell and Tissue Biology,University of California, San Francisco , 2Department of Biology,University of Copenhagen, Denmark, 3National Institute of Nutrition and Seafood Research, Bergen, Norway

Chapters

  • 00:05Title
  • 01:16Adipose Tissue Isolation
  • 02:13Tissue Cutting and Digestion
  • 03:09Preparing Cell Suspension
  • 03:58Plating Cells
  • 04:46Differentiate Cells
  • 05:47Results: Browning of Primary Adipocytes
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

主要从小鼠白色脂肪组织中分离出的白色脂肪前体细胞可以分化成浅米色/ BRITE细胞。这里介绍的是一个可靠的细胞模型系统，研究的分子调控“黑”的白色脂肪。

Tags

Beige AdipocytesBrite AdipocytesStromal Vascular FractionPreadipocytesUCP1PPAR-gammaObesity TreatmentCell DifferentiationPrimary Adipocytes

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