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Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model
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Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

DOI:
10.3791/50193-v

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10:11 min

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March 07, 2013

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1Division of Hematology-oncology, Department of Medicine,Medical University of South Carolina

Chapters

  • 00:05Title
  • 01:42Bone Marrow Cell Isolation
  • 04:34Competitive Bone Marrow Transplantation, Sample Collection, and Genomic DNA Isolation
  • 06:25Standard Curve Preparation and Real-time PCR
  • 07:47Representative Donor Cell Engraftment Results
  • 09:46Conclusion

Summary

Automatic Translation

Automatic Translation

Determining donor cell engraftment presents a challenge in mouse bone marrow transplant models that lack well-defined phenotypical markers. We described a methodology to quantify male donor cell engraftment in female transplant recipient mice. This method can be used in all mouse strains for the study of HSC functions.

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Keywords: Quantitative Real-time PCRDonor Cell EngraftmentCompetitive Murine Bone Marrow TransplantationHematopoietic Stem Cell FunctionZfy1Bcl2Flow Cytometry

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