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从手术标本的人类胰岛细胞激光显微切割的改进方法
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Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
Please note that all translations are automatically generated.
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从手术标本的人类胰岛细胞激光显微切割的改进方法
DOI:
10.3791/50231-v
•
09:43 min
•
January 06, 2013
•
Dorothée Sturm
2
,
Lorella Marselli
,
Florian Ehehalt
2
,
Daniela Richter
,
Marius Distler
,
Stephan Kersting
2
,
Robert Grützmann
,
Krister Bokvist
,
Philippe Froguel
,
Robin Liechti
,
Anne Jörns
,
Paolo Meda
,
Gustavo Bruno Baretton
,
Hans-Detlev Saeger
,
Anke M. Schulte
,
Piero Marchetti
,
Michele Solimena
1
Molecular Diabetology
,
Paul Langerhans Institute Dresden
,
2
Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus
,
University of Technology Dresden
,
3
Department of Endocrinology and Metabolism
,
Metabolic Unit University of Pisa
,
4
Labs DC0522
,
Lilly Corporate Center
,
5
Genomics
,
Faculty of Medicine Imperial College London
,
6
Vital-IT
,
SIB Swiss Institute of Bioinformatics
,
7
Clinical Biochemistry
,
Hannover Medical School
,
8
Cell Physiology and Metabolism, Medical School
,
University of Geneva
,
9
Department of Pathology, University Hospital Carl Gustav Carus
,
University of Technology Dresden
,
10
R&D DIAB Division / Translational Medicine
,
Sanofi-Aventis
Chapters
00:00
Title
00:53
Freezing of Human Pancreatic Tissue
01:39
Preparation of Cryosections
03:08
Dehydration of Cryosections
04:46
Laser Microdissection of Beta-cells
Summary
Automatic Translation
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Automatic Translation
激光显微切割是一种技术,使选定单元格的恢复微量的实质。在这里,我们描述了一个协议,收购手术标本用于转录组研究人类胰岛。我们的协议提高了内在的自发荧光的人类β细胞,从而有利于他们的收藏。
Tags
Laser Microdissection
Pancreatic Islets
Transcriptomics
RNA Preservation
Cryosectioning
Autofluorescence
PALM MicroBeam
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