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从手术标本的人类胰岛细胞激光显微切割的改进方法
JoVE Journal
Medicine
Author Produced
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JoVE Journal Medicine
Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
DOI:

09:43 min

January 06, 2013

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Chapters

  • 00:00Title
  • 00:53Freezing of Human Pancreatic Tissue
  • 01:39Preparation of Cryosections
  • 03:08Dehydration of Cryosections
  • 04:46Laser Microdissection of Beta-cells

Summary

Automatic Translation

激光显微切割是一种技术,使选定单元格的恢复微量的实质。在这里,我们描述了一个协议,收购手术标本用于转录组研究人类胰岛。我们的协议提高了内在的自发荧光的人类β细胞,从而有利于他们的收藏。

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