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1Molecular Diabetology,Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus,University of Technology Dresden, 3Department of Endocrinology and Metabolism,Metabolic Unit University of Pisa, 4Labs DC0522,Lilly Corporate Center, 5Genomics,Faculty of Medicine Imperial College London, 6Vital-IT,SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry,Hannover Medical School, 8Cell Physiology and Metabolism, Medical School,University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus,University of Technology Dresden, 10R&D DIAB Division / Translational Medicine,Sanofi-Aventis
Chapters
- 00:00Title
- 00:53Freezing of Human Pancreatic Tissue
- 01:39Preparation of Cryosections
- 03:08Dehydration of Cryosections
- 04:46Laser Microdissection of Beta-cells
Microdisección por láser es una técnica que permite la recuperación de células seleccionados a partir de cantidades mínimas de parénquima. Aquí se describe un protocolo para la obtención islotes pancreáticos humanos a partir de especímenes quirúrgicos para su uso para transcriptomic estudios. Nuestro protocolo mejora la autofluorescencia intrínseca de las células beta humanas, lo que facilita su recogida.
