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JoVE Journal Medicine
Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
 
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Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Article DOI: 10.3791/50238-v • 15:19 min • January 12th, 2013
January 12th, 2013 •
*1, *1, 1, 1, 1, 1, 1, 2, 3, 4, 1, 1
1Umeå Centre for Molecular Medicine, Umeå University, 2Cell Transplant Center, Diabetes Research Institute, University of Miami,, 3EMBL-CRG Systems Biology Program, Centre for Genomic Regulation, Catalan Institute of Research and Advanced Studies, 4Dept. of Computing Science, Umeå University
* These authors contributed equally

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We describe the adaptation of optical projection tomography (OPT)1 to imaging in the near infrared spectrum, and the implementation of a number of computational tools. These protocols enable assessments of pancreatic β-cell mass (BCM) in larger specimens, increase the multichannel capacity of the technique and increase the quality of OPT data.

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Keywords: Near Infrared Optical Projection Tomography Beta-cell Mass Distribution Diabetes Research Pancreatic Tissue Imaging Multi-channel Imaging Computational Tools Center Of Mass Positioning Post-alignment Tuning Intensity Equalization Sample Holder Islet Transplantation
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