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Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [¹³C]-octanoic Acid Breath Test

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JoVE Journal Medicine

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [¹³C]-octanoic Acid Breath Test

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [¹³C]-octanoic Acid Breath Test

DOI:

10.3791/50301-v

•

12:24 min

•

March 23, 2013

•

Christopher T. Creedon, Pieter-Jan Verhulst, Kyoung M. Choi, Jessica E. Mason, David R. Linden, Joseph H. Szurszewski, Simon J. Gibbons, Gianrico Farrugia

¹Enteric Neuroscience Program, Department of Physiology and Biomedical Engineering,Mayo Clinic

Chapters

00:02Title
00:17Introduction
01:33Basic Principals and Experimental Set-up
03:54Training and habituation of the mice
05:32Preparation of the Test Meal
06:37Starting the Experiment
07:33Experimental Procedures
10:14Calculating the Kinetics of Gastric Emptying
11:24Concluding Remarks

Summary

Automatic Translation

Determination of gastric emptying with a non-invasive [¹³C]-octanoic acid breath test for tracking gastroparesis in female NOD LtJ mice.

Tags

[13C]-octanoic Acid Breath Test Non-obese Diabetic Mice Type 1 Diabetes Gastroparesis Longitudinal Study Carbon Isotope Analyzer

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