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Cell Co-culture Patterning Using Aqueous Two-phase Systems
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Bioengineering
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JoVE Journal Bioengineering
Cell Co-culture Patterning Using Aqueous Two-phase Systems

Cell Co-culture Patterning Using Aqueous Two-phase Systems

DOI:
10.3791/50304-v

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10:11 min

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March 26, 2013

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, , ,
1Department of Biomedical Engineering,University of Michigan , 2Department of Macromolecular Science and Engineering,University of Michigan

Chapters

  • 00:05Title
  • 01:32Phase System Characterization: Determining Thresholds for Phase Separation
  • 03:22Configuration 1: Exclusion Patterning of HeLa Cells
  • 05:24Configuration 2: Island Patterning
  • 06:41Configuration 3: Exclusion / Island Patterned Co-Cultures
  • 08:08Results: Cell Patterning with the Aqueous Two-phase System
  • 09:47Conclusion

Summary

Automatic Translation

Automatic Translation

Aqueous two-phase systems were used to simultaneously pattern multiple populations of cells. This fast and easy method for cell patterning takes advantage of the phase separation of aqueous solutions of dextran and polyethylene glycol and the interfacial tension that exists between the two polymer solutions.

Tags

Cell PatterningAqueous Two-phase SystemsDextranPolyethylene GlycolCell Co-cultureCell Migration Assays

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