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利用双水相系统的细胞共培养图案
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Cell Co-culture Patterning Using Aqueous Two-phase Systems
DOI:

10:11 min

March 26, 2013

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Chapters

  • 00:05Title
  • 01:32Phase System Characterization: Determining Thresholds for Phase Separation
  • 03:22Configuration 1: Exclusion Patterning of HeLa Cells
  • 05:24Configuration 2: Island Patterning
  • 06:41Configuration 3: Exclusion / Island Patterned Co-Cultures
  • 08:08Results: Cell Patterning with the Aqueous Two-phase System
  • 09:47Conclusion

Summary

Automatic Translation

双水相系统,用于同时图案多个的细胞群体。此快速和容易的方法,细胞图案利用葡聚糖和聚​​乙二醇的水溶液,和两者之间的聚合物溶液中存在的界面张力的相分离。

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