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In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer
JoVE Journal
Medicine
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JoVE Journal Medicine
In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer
DOI:

08:55 min

February 22, 2013

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Chapters

  • 00:05Title
  • 01:22Preparation of Surgical Tubing, Vascular Catheters and Chest Wall Window
  • 02:09Tracheostomy
  • 02:56Venous Catheterization
  • 03:43Intravital Mouse Lung Microscopy Surgery
  • 05:54Measurement of the Pulmonary Endothelial Surface Layer Thickness
  • 07:06Representative ESL Measurement Results
  • 08:31Conclusion

Summary

Automatic Translation

The endothelial glycocalyx/endothelial surface layer is ideally studied using intravital microscopy. Intravital microscopy is technically challenging in a moving organ such as the lung. We demonstrate how simultaneous brightfield and fluorescent microscopy may be used to estimate endothelial surface layer thickness in a freely-moving in vivo mouse lung.

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