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在活体小鼠的血管炎症和血栓形成的异型血小板中性粒细胞被激活的内皮细胞相互作用对实时成像
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
DOI:

11:18 min

April 02, 2013

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Chapters

  • 00:05Title
  • 01:20Intravital Microscope and Cremaster Muscle Preparation for Intravital Microscropy
  • 02:30Intravital Microscopy for TNFα-induced Vascular Inflammation
  • 03:33Data Analysis for the Venular Inflammation Model
  • 06:06Intravital Microscopy for Laser-induced Arteriolar Thrombosis
  • 06:58Data Analysis for the Arteriolar Thrombosis Model
  • 08:20Results: Representative Heterotypic Interactions of Platelets and Neutrophils during TNFα-induced Vascular Inflammation and Platelet Thrombus at the Site of Laser-induced Arteriolar Injury in Live Mice
  • 10:52Conclusion

Summary

Automatic Translation

在这里,我们报告荧光活体显微镜可视化激活的内皮细胞在活体小鼠的血管炎症和血栓形成的异型血小板中性粒细胞相互作用的实验技术。显微技术将是很有价值的研究血管疾病的分子机制和病理生理条件下测试药物制剂。

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