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A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
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Biology
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JoVE Journal Biology
A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

DOI:
10.3791/50330-v

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07:07 min

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May 10, 2013

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1Division of Nephrology-Hypertension, Department of Medicine,University of California, San Diego , 2San Diego VA Healthcare System

Chapters

  • 00:05Title
  • 01:39Preparation of FITC-inulin Injection Solution
  • 03:10Injection and Blood Collection
  • 05:23Preparation of Standards
  • 05:56Results: Ability of the FITC-inulin Single-bolus Injection Technique to Determine Changes in GFR
  • 06:45Conclusion

Summary

Automatic Translation

Automatic Translation

Measurement of glomerular filtration rate (GFR) is the gold standard for kidney function assessment. Here we describe a high-throughput method which allows the determination of GFR in conscious mice by using a single bolus injection, determination of fluorescein isothiocyanate (FITC)-inulin in plasma and calculation of GFR by a two-phase exponential decay model.

Tags

Keywords: Glomerular Filtration RateGFRKidney Function AssessmentCreatinineInulinFluorescein IsothiocyanateFITC-inulinTwo-compartment ModelHigh-throughputConscious MiceNon-invasive

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