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Immunology and Infection
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Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means
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Immunology and Infection
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JoVE Journal Immunology and Infection
Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means

Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means

DOI:
10.3791/50342-v

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05:20 min

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March 02, 2013

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, , , ,
1Centro de Biología Celular y Molecular de Enfermedades,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), 2Acharya Nagarjuna University, 3Departamento de Medios y Creativo,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP)

Chapters

  • 00:01Title
  • 01:04Preparation
  • 02:21Loading
  • 02:45Washing
  • 03:16Elution
  • 03:50Loading Repetition
  • 04:44Purity Assessment
  • 05:07Applications

Summary

Automatic Translation

Automatic Translation

The paramagnetic properties of hemozoin are used to isolate late stages of Plasmodium falciparum-infected red blood cells growing in culture. The method is simple and fast and does not affect the subsequent invasive capabilities of the parasites.

Tags

Keywords: Plasmodium FalciparumErythrocytesMagnetic SeparationHaemozoinParamagnetismLate Stage ParasitesCulture Enrichment

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