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Chapters
- 00:05Title
- 01:37Shallow Walled Culture Chamber Assembly
- 02:18Trachea Harvest and Preparation
- 03:36Cilia Imaging
- 05:03Quantitation of Cilia Motility
- 06:59Trachea Cilia Beating in an Adult Mouse
- 07:42Conclusion
An easy and reliable technique for visualizing and quantifying airway cilia motility and cilia generated flow using mouse trachea is described. This technique can be modified to determine how a wide range of factors influence cilia motility, including pharmacological agents, genetic factors, environmental exposures, and/or mechanical factors such as mucus load.
