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Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl
 
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Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl

Article DOI: 10.3791/50375-v • 10:32 min • August 14th, 2013
August 14th, 2013 •
1,2,3,4, 1,2,3, 1,2,3, 1,2,3, 4, 1,2,3, 1,2,3
1U968, Institut National de la Santé et de la Recherche Médicale, 2UMR S 968, Université Pierre et Marie Curie, 3UMR 7210, Centre National de la Recherche Scientifique, 4Départment d'Ophtalmologie, Centre Hospitalier Universitaire de Bordeaux

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We used retinal samples from retinectomy for a transcriptomic analysis of retinal detachment. We developed a procedure that allows RNA conservation between the surgical blocks and the laboratory. We standardized a protocol to purify RNA by cesium chloride ultracentrifugation to assure that the purified RNAs are suitable for microarray analysis.

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Keywords: Retinal Detachment Retinal Pigmented Epithelium Photoreceptor Vitrectomy RNA Extraction Transcriptomics Gene Expression Analysis Inflammation Photoreceptor Degeneration
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