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基因表达谱分析人视网膜手术标本杂志
JoVE Journal
Medicine
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JoVE Journal Medicine
Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl
DOI:

10:32 min

August 14, 2013

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Chapters

  • 00:05Title
  • 01:32Preparation of Sample Tubes for jouRNAl
  • 02:29Sample Collection
  • 03:28RNA Purification
  • 08:09Representative Analysis of Retinal Tissue-derived RNA
  • 09:51Conclusion

Summary

Automatic Translation

我们使用了视网膜从retinectomy样本为转录组分析视网膜脱离。我们开发了一种程序,允许RNA保护手术块和实验室之间。我们的标准化的协议通过氯化铯超速离心法,以保证纯化的RNA是适合于微阵列分析中纯化RNA。

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