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Biology
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重组脂膜的结构和功能研究的钾离子通道
JoVE Journal
Biology
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JoVE Journal
Biology
Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
Please note that all translations are automatically generated.
Click here for the English version.
重组脂膜的结构和功能研究的钾离子通道
DOI:
10.3791/50436-v
•
10:22 min
•
July 13, 2013
•
Sungsoo Lee*
1
,
Hui Zheng*
1
,
Liang Shi
,
Qiu-Xing Jiang
1
Department of Cell Biology
,
University of Texas Southwestern Medical Center at Dallas
Chapters
00:05
Title
01:27
Liposome Preparation and Detergent-induced Fusion of Vesicles
04:15
Removal of the Detergents to Form Proteoliposomes
06:28
Functional Study of the Ion Channel Activities in Black Lipid Membranes
08:49
Results: Two-Dimensional Crystallization of KvAP in Membranes
09:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
完整的原型脂膜的电压门控钾离子通道进入重组程序中描述的。重组的渠道都适合生化检测,电气录音,配体筛选和电子晶体学研究。这些方法可以有其它膜蛋白的结构和功能研究的一般应用。
Tags
Kv Channel
Lipid Reconstitution
Membrane Proteins
Voltage-gated Potassium Channel
Lipid-protein Interaction
Membrane Systems
Detergent-induced Fusion
2D Crystals
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