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Immunology and Infection
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4D Multimodalitet Imaging af<em> Citrobacter rodentium</em> Infektioner i mus
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
4D Multimodality Imaging of Citrobacter rodentium Infections in Mice

4D Multimodalitet Imaging af<em> Citrobacter rodentium</em> Infektioner i mus

DOI:
10.3791/50450-v

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12:38 min

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August 13, 2013

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, , , ,
1MRC Centre for Molecular Bacteriology and Infection, Division of Cell & Molecular Biology,Imperial College London, 2Preclinical Imaging,Caliper- A PerkinElmer Company

Chapters

  • 00:05Title
  • 01:21Preparing the Bacteria
  • 02:44Infection of Mice with Bioluminescent C. rodentium
  • 04:09Daily Composite 3D Diffuse Light Imaging Tomography with Integrated μCT Imaging (DLIT-μCT) of Infected Mice
  • 06:153D Reconstruction of DLIT-μCT Imaging Data
  • 09:29Generation of a 4D Movie of C. rodentium Infection
  • 10:24Monitored Infection with 5 x 109 cfu C. rodentium, Strain ICC180
  • 12:16Conclusion

Summary

Automatic Translation

Automatic Translation

Multi-modalitet billeddannelse er en værdifuld metode til at studere bakteriel kolonisering i små dyremodeller. Denne protokol skitserer infektion af mus med bioluminiscerende<em> Citrobacter rodentium</em> Og den langsgående overvågning af bakteriel kolonisering med komposit 3D diffust lys imaging tomografi med μCT billeddannelse for at skabe en 4D film på<em> C. rodentium</em> Infektion.

Tags

4D Multimodality ImagingCitrobacter RodentiumBioluminescenceDLIT-μCTLongitudinal MonitoringBacterial InfectionColitisMouse ModelIVIS Spectrum CT3D Optical-CT Co-registrationLow-dose μCTBacterial Colonization Dynamics

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