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Tissue Engineering of a Human 3D in vitro Tumor Test System
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Tissue Engineering of a Human 3D in vitro Tumor Test System

Tissue Engineering of a Human 3D in vitro Tumor Test System

DOI:
10.3791/50460-v

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11:12 min

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August 06, 2013

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, , , , , ,
1Department of Tissue Engineering and Regenerative Medicine,University Hospital Würzburg

Chapters

  • 00:05Title
  • 02:16Decellularization of the BioVaSc
  • 03:52Isolation of Primary Human Dermal Microvascular Endothelial Cells (mvECs) and Fibroblasts
  • 05:50Tumor Test System A. Static Culture
  • 07:36Tumor Test System B. Dynamic Culture
  • 08:45Results: Immunohistological Characterization of 3D Tumor Models
  • 10:40Conclusion

Summary

Automatic Translation

Automatic Translation

Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.

Tags

Keywords: 3D Tumor ModelCancer ResearchMalignant Peripheral Nerve Sheath Tumors (MPNSTs)Decellularized Biological ScaffoldBioVaScStatic CultureDynamic BioreactorCell-cell InteractionsIn Vitro Model

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