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糖原生化滴定<em>在体外</em

JoVE Journal
Biology

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JoVE Journal Biology

Biochemical Titration of Glycogen In vitro

Please note that all translations are automatically generated. Click here for the English version.

糖原生化滴定<em>在体外</em

DOI:

10.3791/50465-v

•

07:16 min

•

November 24, 2013

•

Joffrey Pelletier, Grégory Bellot, Jacques Pouysségur, Nathalie M. Mazure

¹Institute for Research on Cancer and Ageing of Nice, UMR CNRS 7284 – INSERM, U1081 – UNS, Centre Antoine Lacassagne,University of Nice – Sophia Antipolis

Chapters

00:05Title
00:54Cell Lysis
02:50Hydrolysis of Glycogen
03:55Development and Reading of Fluorescence
05:28Results: Glycogen Quantification and Its Role in Hypoxia
06:49Conclusion

Summary

Automatic Translation

我们在这里描述的糖原体外滴定准确，重现性好，方便的生化方法。这种技术使用了Abcam公司糖原测定试剂盒和基于糖原的连续水解的荧光成葡萄糖和葡萄糖滴定。

Tags

Glycogen Biochemical Titration In Vitro Glucose Glucoamylase OxiRed Probe Fluorescence Cell Line CCL39 LS174 Normoxia Hypoxia

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