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Bestemmelse af tolerabel fedtsyrer og koleratoxin Koncentrationer anvendelse af humant tarmepitelceller og BALB / c musemakrofager
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages
DOI:

09:39 min

May 30, 2013

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Chapters

  • 00:05Title
  • 00:59Tissue Culture
  • 02:24Fatty Acid Treatment
  • 04:12Cholera Toxin Treatment
  • 05:38MTT Assay
  • 06:47Results: Effects of Fatty Acids and Cholera Toxin on Cell Survival
  • 09:16Conclusion

Summary

Automatic Translation

Vi satte os for at bestemme acceptable koncentrationer af tre fedtsyrer (oliesyre, linolsyre og linolensyre), og kolera toksin, der ikke væsentligt negativ indflydelse celleoverlevelse ved at opløse fedtsyrerne og toksin og bruge dem i celle overlevelse assays.

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