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Den<em> I ovo</em> CAM-assay som et Xenograftmodel til Sarkom
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JoVE Journal Medicine
The In ovo CAM-assay as a Xenograft Model for Sarcoma

Den<em> I ovo</em> CAM-assay som et Xenograftmodel til Sarkom

DOI:
10.3791/50522-v

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12:44 min

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July 17, 2013

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, , , , , ,
1Department of Orthopaedic Surgery and Traumatology,Ghent University Hospital, 2Department of Radiation Oncology and Experimental Cancer Research,Ghent University, 3Department of Virology, Parasitology, and Immunology,Ghent University, 4Pathlicon

Chapters

  • 00:05Title
  • 02:19Preparation of Tumor-derived Single Cell Suspensions
  • 03:59Opening of the Eggs
  • 05:21Inoculation Procedure
  • 06:23Imaging and Harvesting of the CAM
  • 07:20Results: Evaluation of the CAM
  • 11:30Conclusion

Summary

Automatic Translation

Automatic Translation

Den<em> In ovo</em> Chorioallantoide membran (CAM) er podet med friske sarkom-afledte tumorvæv, deres encellede suspensioner og permanente og forbigående fluorescensmærkede etablerede sarkom cellelinjer. Modellen anvendes til at studere graft-(rentabilitet, Ki67 proliferationsindeks, nekrose, infiltration) og vært (fibroblast infiltration, vaskulær indvækst) adfærd.

Tags

SarcomaXenograft ModelChick Chorioallantoic Membrane (CAM)In OvoCell LinesSaos-2SW1353Fluorescent LabelingGraft BehaviorHost ResponseKi67 Proliferation IndexNecrosisVascular IngrowthECM Gel

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