/

/

联体小鼠：一个详细的协议

JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine

Parabiosis in Mice: A Detailed Protocol

Please note that all translations are automatically generated. Click here for the English version.

联体小鼠：一个详细的协议

DOI:

10.3791/50556-v

•

07:56 min

•

October 06, 2013

•

Paniz Kamran², Konstantina-Ioanna Sereti², Peng Zhao², Shah R. Ali, Irving L. Weissman, Reza Ardehali²

¹Department of Medicine-Division of Cardiology,University of California, Los Angeles, ²Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research,University of California, Los Angeles, ³Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine

Chapters

00:05Title
01:06Surgical Preparations
02:22Parabiosis Surgery
04:35Post-operative Recovery
05:23Confirmation and Reverse Procedure
07:05Anticipated Outcome Using GFP
07:36Conclusion

Summary

Automatic Translation

联体接合的两个生物体导致的共享循环系统的发展。在这个协议中，我们描述了手术步骤，以形成一个野生型小鼠和组成型表达GFP的小鼠之间的联体连接。

Tags

Parabiosis Mice Surgical Union Blood Circulation Microvasculature Immune Reaction GFP Expressing Mouse Wild Type Mouse Blood Chimerism Flow Cytometry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

特里尔社会压力诱发心理应激的测试协议

持续时间较长的小鼠全身热疗协议

人体胰腺的收集协议

颈动脉输液的紫杉烷类化合物在小鼠体内的药代动力学和药效学分析

一个简单的临界尺寸股骨缺损模型小鼠

异位自体肾移植的猪模型：有步骤，分步协议

一种用于兔视网膜下手术分步协议

小鼠腹腔粘连的创建

详细的汗渍监控协议使用一种新的，小的，无线设备

神经外科危重患者生理参数采集与分析的详细方案

Read Article