Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Localization and Relative Quantification of…
JoVE Journal
Bioengineering

This content is Open Access.

JoVE Journal Bioengineering
Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry
 
Click here for the English version

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

Article DOI: 10.3791/50566-v • 14:09 min • December 12th, 2013
December 12th, 2013 •
*1, *2, 3, 1, 1, 3, 1
1Laboratoire Matière et Systèmes Complexes (MSC), CNRS/Université Paris Diderot, 2ImagoSeine BioImaging Core Facility, Institut Jacques Monod, CNRS/Université Paris Diderot, 3Laboratoire d'Immunopathologie et Chimie Thérapeutique, CNRS/Institut de Biologie Moléculaire et Cellulaire
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

In this study, the main purpose was to monitor the cellular uptake and eventual exocytosis of carbon nanotubes (CNTs). From this perspective, we proposed here a unique method using multispectral imaging flow cytometry allowing a quantification and localization of CNTs in a statistically relevant number of cells.

Tags

Keywords: Carbon Nanotubes CNTs Localization Quantification Multispectral Imaging Flow Cytometry ImageStream Light Absorption Light Scattering Fluorescence Pixel-by-pixel Analysis Nanomaterials Cell-nanomaterial Interactions
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter