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Bioengineering

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JoVE Journal Bioengineering
Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System
 

Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion

Article DOI: 10.3791/50604-v • 10:30 min • September 4th, 2013
September 4th, 2013 •
1, 2, 1,2
1Department of Chemical and Biomolecular Engineering, Russ College of Engineering and Technology, Ohio University, 2Biomedical Engineering Program, Ohio University

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Summary
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Dual camera emission splitting systems for two-color fluorescence microscopy generate real-time image sequences with exceptional optical and temporal resolution, a requirement of certain live cell assays including parallel plate flow chamber adhesion assays. When software is employed to merge images from simultaneously acquired emission channels, pseudocolored image sequences are produced.

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Keywords: Dual Camera Emission Splitting System Multi-color Immunofluorescence Microscopy Parallel Plate Flow Chamber Real-time Live Cell Analysis Fluorescently Labeled Cells Cell Adhesion Cancer Metastasis
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