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Immunology and Infection
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Optimerede protokoller til<em> Mycobacterium leprae</em> Strain Management: Frosne Stock bevarelse og vedligeholdelse i Atymiske Nude Mus
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice

Optimerede protokoller til<em> Mycobacterium leprae</em> Strain Management: Frosne Stock bevarelse og vedligeholdelse i Atymiske Nude Mus

DOI:
10.3791/50620-v

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10:32 min

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March 23, 2014

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, , , , , ,
1Department of Pathology,Instituto Lauro de Souza Lima (ILSL), 2Laboratory Animal House,Instituto Lauro de Souza Lima (ILSL), 3Department of Microbiology,Instituto Lauro de Souza Lima (ILSL), 4Department of Pharmacology,Instituto Lauro de Souza Lima (ILSL)

Chapters

  • 00:00Title
  • 00:20Preparation of Mycobacterium leprae Suspension
  • 04:24Cold Ziehl-Neelsen Stain
  • 06:06Viability Determination
  • 07:48Freezing/Thawing M. leprae Suspension
  • 09:08Mouse Inoculation
  • 09:35Results

Summary

Automatic Translation

Automatic Translation

Mycobacterium leprae, det agens af spedalskhed, ikke vokser in vitro. Vi beskriver en nem at følge protokollen til at forberede en bacillær suspension til at sikre opretholdelsen af store mængder M. leprae for en lang række applikationer. Protokoller til formering af mus footpad podning, evaluering af levedygtighed, nedfrysning og optøning bacillær bestand er beskrevet i detaljer.

Tags

Mycobacterium LepraeLeprosyAthymic Nude MiceBacilli PurificationTrypsinZiehl-Neelsen StainingBacillary CountingFrozen Stock PreservationStrain Maintenance

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