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Automatiserede High-throughput Behavioral Analyser i zebrafisk Larver
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Automated High-throughput Behavioral Analyses in Zebrafish Larvae
DOI:

09:28 min

July 04, 2013

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Chapters

  • 00:05Title
  • 01:48Collection of Zebrafish Embryos and Raising Larvae
  • 03:08Preparing Molds for Behavioral Analyses
  • 04:02Preparation of Imaging Cabinet
  • 04:39Image Capture
  • 06:08Image Analysis
  • 07:31Results: Example of Behaviors that Can Be Quantified in the High-throughput Assay
  • 08:57Conclusion

Summary

Automatic Translation

Vores laboratorium udviklet en ny high-throughput automatiseret billeddannende system, der er nyttige til påvisning af flere forskellige adfærd i 7 dage gammel zebrafisk larver. Systemet kan bruges til at detektere små ændringer i adfærd, når æggene er blevet udsat for miljøgiftstoffer eller lægemidler.

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