Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Generation and 3-Dimensional Quantitation of…
JoVE Journal
Medicine

This content is Open Access.

JoVE Journal Medicine
Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography
 

Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography

Article DOI: 10.3791/50627-v • 11:45 min • May 26th, 2015
May 26th, 2015 •
1, 1, 1, 1, 1, 1, 1, 1
1University/ BHF Centre for Cardiovascular Science, University of Edinburgh, The Queen's Medical Research Institute

Chapters

Summary
Automatic Translation

Ex vivo analysis of arterial lesions from animal models of cardiovascular disease classically relies on histological and immunohistochemical techniques. These provide 2-dimensional measurements in 3-dimensional lesions. This manuscript describes the generation of arterial lesions for quantitative analysis in 3-dimensions using optical projection tomography.

Tags

Keywords: Optical Projection Tomography 3D Quantitation Arterial Lesions Mice Atherosclerosis Neointimal Lesions Wire-insertion Ligation ApoE-deficient Histology Immunohistochemistry Vascular Remodeling
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter