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Immunology and Infection

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JoVE Journal Immunology and Infection
Concurrent Quantification of Cellular and Extracellular Components of Biofilms
 
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Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Article DOI: 10.3791/50639-v • 10:18 min • December 10th, 2013
December 10th, 2013 •
1, 1, 1,3, 2
1Department of Dental Materials, College of Dentistry, University of Oklahoma Health Sciences Center, 2Department of Biostatistics and Epidemiology, College of Public Health, University of Oklahoma Health Sciences Center, 3Department of Biological Sciences, School of Mathematics and Natural Sciences, The Copperbelt University

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A protocol for the concurrent quantification and comparison of three cellular and extracellular components within biofilms is presented. The methodology involves the use of confocal laser scanning microscopy, biofilm structural analysis and visualization software, and statistical analysis software.

Tags

Biofilm Confocal Laser Scanning Microscopy Extracellular Polymeric Substances Proteins Nucleic Acids Streptococcus Mutans Resin Composites Mouthwashes Biovolume Mean Biofilm Thickness
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