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Viability Assays for Cells in Culture
JoVE Journal
Biology
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JoVE Journal Biology
Viability Assays for Cells in Culture
DOI:

12:03 min

January 20, 2014

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Chapters

  • 00:05Title
  • 01:38Luminescent ATP Assay
  • 04:05Infrared Assay
  • 08:11Data Acquisition and Analysis for the Infrared Assays
  • 09:22Results: Three Measurements Are Better Than One
  • 11:23Conclusion

Summary

Automatic Translation

Therapeutic compounds are often first examined in vitro with viability assays. Blind cell counts by a human observer can be highly sensitive to small changes in cell number but do not assess function. Computerized viability assays, as described here, can assess both structure and function in an objective manner.

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