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1Center for Environmental Medicine, Asthma, and Lung Biology,The University of North Carolina at Chapel Hill, 2Department of Pediatrics,The University of North Carolina at Chapel Hill, 3Pulmonary Diseases and Critical Care,The University of North Carolina at Chapel Hill, 4Curriculum in Toxicology,The University of North Carolina at Chapel Hill
Chapters
- 00:05Title
- 02:05Obtaining Biopsy of Human NECs
- 03:37Seeding NECs on Plastic
- 04:41Expanding the Cells in Flasks
- 06:00Seeding Cells on Tissue Culture Inserts
- 07:53Establishing Air Liquid Interface (ALI)
- 08:35ALI Culture of NECs Produces a Differentiated Epithelium
- 09:43Conclusion
Nasal epithelial cells, obtained through superficial scrape biopsy of human volunteers, are expanded and transferred onto tissue culture inserts. Upon reaching confluency, cells are grown at air liquid interface, yielding cultures of ciliated and non-ciliated cells. Differentiated nasal epithelial cell cultures provide viable experimental models for studying the respiratory mucosa.
