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Fremstilling af rekombinant arenavirus for Vaccine Development i FDA-godkendte Vero celler
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells

Fremstilling af rekombinant arenavirus for Vaccine Development i FDA-godkendte Vero celler

DOI:
10.3791/50662-v

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10:03 min

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August 01, 2013

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, , ,
1Department of Microbiology and Immunology,University of Rochester School of Medicine and Dentistry, 2Departments of Immunology and Microbial Sciences,The Scripps Research Institute

Chapters

  • 00:05Title
  • 01:36Arenavirus Rescue Transfection
  • 04:41Confirmation of Recombinant Arenavirus Rescue
  • 07:11r3LCMV and r3Candid#1 Viral Rescue
  • 08:06Results: Arenavirus in Vero Cells
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

Redning af rekombinante arenaviruses fra klonede cDNA'er, en tilgang kaldet reverse genetik, giver forskerne at undersøge betydningen af ​​specifikke virale genprodukter, samt bidrag af deres forskellige specifikke domæner og restprodukter til mange forskellige aspekter af biologi arenavirus . Ligeledes omvendt genetik teknikker i FDA-godkendte cellelinier (Vero) for udvikling af vacciner giver nye muligheder til generering af effektive og sikre vacciner til bekæmpelse af humane patogene arenaviruses.

Tags

Keywords: Recombinant ArenavirusVaccine DevelopmentFDA-approved Vero CellsArenavirus Reverse GeneticsCell-based Arenavirus Minigenome SystemsRecombinant Infectious ArenavirusesTrisegmented ArenavirusesSingle-cycle Infectious ArenavirusesReporter GenesPlasmid-based Reverse GeneticsRodent Cell LinesFDA-licensed VaccineVaccine Vectors

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