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Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies
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Bioengineering
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JoVE Journal Bioengineering
Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies

Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies

DOI:
10.3791/50678-v

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13:02 min

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July 15, 2013

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, , , ,
1Department of Electrical and Computer Engineering,University of California, Davis , 2Department of Chemical Engineering and Materials Science,University of California, Davis , 3Department of Biomedical Engineering,University of California, Davis

Chapters

  • 00:05Title
  • 01:49Nanoporous Gold Fabrication
  • 06:43Cell Culture
  • 08:01Cell and Material Analysis
  • 10:15Quantification of Nanostructure and Cell-Material Interactions of Micropatterned Nanoporous Gold Films
  • 12:07Conclusion

Summary

Automatic Translation

Automatic Translation

We report on techniques to micropattern nanoporous gold thin films via stencil printing and photolithography, as well as methods to culture cells on the microfabricated patterns. In addition, we describe image analysis methods to characterize morphology of the material and the cultured cells using scanning electron and fluorescence microscopy techniques.

Tags

Nanoporous GoldMicrofabricationNanomaterialsCell-material InteractionBiosensorsThin FilmsPhotolithographyStencil MasksSelf-assemblySurface AreaElectrical ConductivityCatalytic ActivityBioconjugationImmunostainingImage Processing

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