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Micron-skala Resolution Optical Tomography av hele mus hjerner med Confocal Lett Sheet Mikros
This content is Free Access.
1European Laboratory for Non-linear Spectroscopy (LENS), 2Integrated Research Centre,University Campus Bio-medico of Rome, 3DAEMI,University of Cassino, 4National Institute of Optics (CNR-INO), 5Allen Institute for Brain Science, 6Department of Physics,University of Florence, 7ICON Foundation, Sesto Fiorentino, Italy
Chapters
- 00:05Title
- 01:22Dehydration and Clearing
- 02:37Specimen Mounting
- 03:33Tomography Preparation
- 04:26Tomography Acquisition
- 05:37Stitching and Visualization
- 07:35Results: Micron-scale Resolution Optical Tomography
- 08:54Conclusion
I denne artikkelen beskriver vi full eksperimentell prosedyre for å rekonstruere, med høy oppløsning, den fine hjernen anatomi fluorescensmerkede mus hjerner. Den beskrevne protokollen omfatter prøveopparbeidelse og clearing prøven monterings for bildebehandling, data post-prosessering og multi-skala visualisering.
