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Isolering af fedtvæv immunceller
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Adipose Tissue Immune Cells
DOI:

07:09 min

May 22, 2013

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Chapters

  • 00:05Title
  • 01:11Harvesting and Preparation of Adipose Tissue
  • 02:06Collagenase Digestion
  • 03:19Staining of Cell Surface Antigens
  • 04:23FACS Analysis
  • 05:13Representative Results
  • 06:54Conclusion

Summary

Automatic Translation

Fedtvæv (AT) er et site for intens immun celle aktivering og interaktion. Næsten alle celler i immunsystemet er til stede i AT og deres forhold ændres ved fedme. Korrekt isolation, kvantificering og karakterisering af AT immun cellepopulationer er afgørende for at forstå deres rolle i immunometabolic sygdom.

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