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Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels
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Neuroscience
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JoVE Journal Neuroscience
Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels

Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels

DOI:
10.3791/50782-v

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11:34 min

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December 24, 2013

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, ,
1Baker Laboratory of Pharmacology, Department of Pharmacology, Kirksville College of Osteopathic Medicine,AT Still University of Health Sciences

Chapters

  • 00:05Title
  • 01:35Animal Preparation and Labeling
  • 02:27Dissection and Neuronal Isolation
  • 07:06Patch-clamp Recording
  • 08:09Immunocytochemistry
  • 09:42Results: Identification of Specific Sensory Neuron Populations and Patch-clamp Recording
  • 11:01Conclusion

Summary

Automatic Translation

Automatic Translation

Afferent sensory neurons signal sensory information from the periphery to the central nervous system. Identifying specific afferent neurons will help in understanding their physiology. We describe a method of retrograde labeling to identify afferent neurons, and study the voltage-gated ion channels in these neurons using patch clamp electrophysiology and immunocytochemistry.

Tags

Keywords: Sensory NeuronsDorsal Root GangliaIon ChannelsMuscle AfferentsRetrograde LabelingDiIPatch Clamp ElectrophysiologyImmunocytochemistryVoltage-dependent Ion ChannelsPeripheral Vascular DiseaseHeart Failure

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