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Preparazione di primaria neuroni per visualizzare neuriti in uno Stato Frozen-idratata Utilizzo Cryo-Electron Positron
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1Department of Molecular Physiology and Biophysics,Baylor College of Medicine, 2Department of Neuroscience,Baylor College of Medicine, 3Department of Neuroscience,University of California at San Diego, 4National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology,Baylor College of Medicine
Chapters
- 00:05Title
- 01:19Preparing Dishes with EM Grids
- 03:43Preparing and Plating Primary Neurons on EM Grids
- 04:45Vitrifying Neurons on EM Grids
- 08:05Results: Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography
- 09:32Conclusion
Per conservare processi neuronali per l'analisi ultrastrutturale, si descrive un protocollo per la placcatura di neuroni primari su Electron griglie microscopia seguite da congelamento flash, ottenendo campioni sospesi in uno strato di ghiaccio vitreo. Questi campioni possono essere esaminate con un microscopio crio-elettroni per visualizzare le strutture in scala nanometrica.
