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分离,培养和成像胎儿胰腺细胞集群
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters
DOI:

08:09 min

May 18, 2014

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Chapters

  • 00:05Title
  • 01:20Dissection of Human Fetal Pancreas
  • 03:48Immunofluorescence and Maturation of Islet Cell Clusters
  • 05:36Results: Isolated, Cultured, and Imaged Human Fetal Pancreatic Cell Clusters
  • 07:34Conclusion

Summary

Automatic Translation

一个协议隔离开来,从人胎儿胰腺细胞衍生的文化,形象胰岛细胞团(IC卡)中说明。该方法的详细信息需要从组织,文化为单层或悬浮液中的聚集和图像增殖的标记和胰腺细胞命运的决定产生IC卡的步骤。

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