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分离,培养和成像胎儿胰腺细胞集群
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters
Please note that all translations are automatically generated.
Click here for the English version.
分离,培养和成像胎儿胰腺细胞集群
DOI:
10.3791/50796-v
•
08:09 min
•
May 18, 2014
•
Ana D. Lopez
,
Ayse G. Kayali
,
Alberto Hayek
,
Charles C. King
1
Pediatric Diabetes Research Center, Department of Pediatrics
,
University of California, San Diego
Chapters
00:05
Title
01:20
Dissection of Human Fetal Pancreas
03:48
Immunofluorescence and Maturation of Islet Cell Clusters
05:36
Results: Isolated, Cultured, and Imaged Human Fetal Pancreatic Cell Clusters
07:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一个协议隔离开来,从人胎儿胰腺细胞衍生的文化,形象胰岛细胞团(IC卡)中说明。该方法的详细信息需要从组织,文化为单层或悬浮液中的聚集和图像增殖的标记和胰腺细胞命运的决定产生IC卡的步骤。
Tags
Human Fetal Pancreatic Cell Clusters
Insulin Producing Cells
Endocrine Precursor Cells
HESC
In Vitro Genesis
C-peptide
Pancreatic Development
Pancreatic Endoderm
Pancreatic Markers
Glucagon
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