Journal
/
Biology
/
Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

DOI:
10.3791/50838-v

•

10:08 min

•

November 20, 2013

•

, ,
1Department of Cell and Molecular Biology,Feinberg School of Medicine, Northwestern University, 2IKERBASQUE,Basque Foundation for Science

Chapters

  • 00:05Title
  • 01:34Drosophila S2 Cells
  • 03:19Drosophila Neuron Culture
  • 07:47Visualized Microtuble Transport in Cultured S2 Cells and Neurons
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

Drosophila S2 cells and cultured neurons are great systems for imaging of motor-driven organelle transport in vivo. Here we describe detailed protocols for culturing both cell types, their imaging and analysis of transport.

Tags

Keywords: DrosophilaS2 CellsPrimary NeuronsOrganelle TransportMicrotubulesLive ImagingRNAiCargo TransportAxonal TransportCell Culture

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50838?article_type=v

Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae
check_url/50838?article_type=v

Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons
check_url/50838?article_type=v

Preparation of Drosophila S2 cells for Light Microscopy
check_url/50838?article_type=v

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
check_url/50838?article_type=v

Primary Cell Cultures from Drosophila Gastrula Embryos
check_url/50838?article_type=v

Isolation and Primary Culture of Rat Hepatic Cells
check_url/50838?article_type=v

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis
check_url/50838?article_type=v

Generating a "Humanized" Drosophila S2 Cell Line Sensitive to Pharmacological Inhibition of Kinesin-5
check_url/50838?article_type=v

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
check_url/50838?article_type=v

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Read Article