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Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells (MBC-P) and Biofilm Cells (MBC-B)
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Chapters
- 00:05Title
- 01:03Minimal Bactericidal Concentration of Biofilm Cells (MBC-B)
- 02:40Assay for Live Cells
- 03:34Minimal Bactericidal Concentration of Planktonic Cells (MBC-P)
- 05:22Results: MBC-P and MBC-B Assays
- 06:18Conclusions
This protocol allows for a direct comparison between planktonic and biofilm resistance for a bacterial strain that can form a biofilm in vitro using a 96-well microtiter plate. Planktonic or biofilm bacteria are exposed to serial dilutions of the antimicrobial agent of choice. Viability is assayed by growth on agar plates.
