Journal
/
Neuroscience
/
Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals

Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals

DOI:
10.3791/50884-v

•

09:53 min

•

February 11, 2014

•

, , ,
1CGphiMC, UMR CNRS 5534,University of Lyon 1

Chapters

  • 00:05Title
  • 01:45Spinal Cord Dissection
  • 05:03FP Culture and FP Conditioned Medium Harvesting
  • 06:35Commissural Neuron Dissection and Treatments
  • 08:15Results: Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals
  • 08:58Conclusion

Summary

Automatic Translation

Automatic Translation

The floor plate is a crucial structure of the developing spinal cord, providing diffusible signals to progenitors and differentiating neurons. We describe a method to produce floor-plate conditioned medium, to apply it to fresh spinal cord tissue, and to assess the consequences on proteins of interest by biochemistry.

Tags

Keywords: Floor PlateConditioned MediumSpinal Cord DevelopmentAxon GuidanceMorphogensCommissural AxonsGuidance CuesSignaling Pathways

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50884?article_type=v

A Functional Motor Unit in the Culture Dish: Co-culture of Spinal Cord Explants and Muscle Cells
check_url/50884?article_type=v

Recording Human Electrocorticographic (ECoG) Signals for Neuroscientific Research and Real-time Functional Cortical Mapping
check_url/50884?article_type=v

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue
check_url/50884?article_type=v

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
check_url/50884?article_type=v

Closed-loop Neuro-robotic Experiments to Test Computational Properties of Neuronal Networks
check_url/50884?article_type=v

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue
check_url/50884?article_type=v

Characterizing Multiscale Mechanical Properties of Brain Tissue Using Atomic Force Microscopy, Impact Indentation, and Rheometry
check_url/50884?article_type=v

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium
check_url/50884?article_type=v

Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue
check_url/50884?article_type=v

Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

Read Article