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Observation og kvantificering fibroblast-medieret Fibrin Gel Compaction
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Bioengineering
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JoVE Journal Bioengineering
Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction

Observation og kvantificering fibroblast-medieret Fibrin Gel Compaction

DOI:
10.3791/50918-v

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10:37 min

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January 16, 2014

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1Department of Biomedical Engineering,University of Iowa

Chapters

  • 00:05Title
  • 01:42Fibrin Gel Preparation
  • 04:41Cell Explant Preparation
  • 07:42Time-lapse Imaging and Strain Tracking
  • 09:23Results: Time-lapse Microscopy and Strain Tracking of Fibrin Reorganization
  • 09:58Conclusion

Summary

Automatic Translation

Automatic Translation

Time-lapse mikroskopi og billedbehandling teknikker blev brugt til at observere og analysere fibroblast-medieret gel komprimering og fibrin fiber justering i en miljøkontrolleret bioreaktor over en 48 timers periode.

Tags

Fibrin Gel CompactionFibroblast-mediated ContractionGel Microstructure ReorganizationTime-lapse DIC MicroscopyStrain MappingMechanobiologyCell-matrix InteractionsWound Healing

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