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Immunology and Infection

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JoVE Journal Immunology and Infection
Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation
 

Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation

Article DOI: 10.3791/50926-v • 11:25 min • December 26th, 2013
December 26th, 2013 •
1, 1,2, 1, 1, 1
1Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes (URMITE), CNRS UMR 7278, INSERM U1095, Aix Marseille Université, 2Service de médecine interne, post-urgence et thérapeutique, Hôpital de la Timone

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This protocol describes the use of whole-cell MALDI-TOF mass spectrometry on eukaryotic cells. Here, we illustrate the accuracy of this technique by analyzing the multiple activation states of macrophages in response to their microenvironment.

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Keywords: MALDI-TOF Mass Spectrometry Macrophage Activation Whole-cell Analysis Proteomic Fingerprinting Cell Identification Diagnostic Applications
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