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Journal / Bioengineering / Cell Patterning on Photolithographically Defined …
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Bioengineering

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JoVE Journal Bioengineering
Cell Patterning on Photolithographically Defined Parylene-C
 

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates

Article DOI: 10.3791/50929-v • 07:19 min • March 7th, 2014
March 7th, 2014 •
1, 2, 3, 1, 3
1Centre for Integrative Physiology, School of Biomedical Sciences, The University of Edinburgh, 2Edinburgh Cancer Research Centre, Institute of Genetics and Molecular Medicine, Western General Hospital, 3School of Engineering, Institute for Integrated Micro and Nano Systems, The University of Edinburgh

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Summary
Automatic Translation

This protocol describes a microfabrication-compatible method for cell patterning on SiO2. A predefined parylene-C design is photolithographically printed on SiO2 wafers. Following incubation with serum (or other activation solution) cells adhere specifically to (and grow according to the conformity of) underlying parylene-C, whilst being repulsed by SiO2 regions.

Tags

Cell Patterning Parylene-C SiO2 Substrates Photolithography Multi-electrode Arrays Lab On A Chip Cell Adhesion Cell-specific Adhesion Molecules Primary Murine Hippocampal Cells HEK 293 Cell Line Human Neuron-like Teratocarcinoma Cell Line Primary Murine Cerebellar Granule Cells Primary Human Glioma-derived Stem-like Cells
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