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Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
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Medicine
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JoVE Journal Medicine
Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

DOI:
10.3791/50957-v

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10:59 min

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November 18, 2013

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, , , , , ,
1Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine,University of Michigan Medical Center, 2Department of Molecular Pathology,University of Yamanashi

Chapters

  • 00:05Title
  • 01:53Rabbit Embryo Collection
  • 03:28Preparation of ZFN mRNA
  • 04:14Embryo Microinjection
  • 06:38Embryo Transfer
  • 08:12Targeted Mutation of ApoCIII
  • 10:07Conclusion

Summary

Automatic Translation

Automatic Translation

Recent development in gene targeting tools makes production of knockout (KO) rabbits possible. In the present work, we generated five Apolipoprotein (Apo) C-III KO rabbits using Zinc Finger Nucleases (ZFN). This work demonstrated that ZFN is a highly efficient method to produce KO rabbits.

Tags

Keywords: Apolipoprotein C-IIIApoCIIIKnockoutRabbitsZinc Finger NucleasesCardiovascular DiseasesLipid MetabolismAtherosclerosis

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