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自主的生物发光哺乳动物细胞连续和实时监测细胞毒性
JoVE Journal
Biology
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JoVE Journal Biology
Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity
DOI:

04:47 min

October 28, 2013

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Chapters

  • 00:05Title
  • 01:11Cell Plating and Experimental Treatment
  • 02:22Imaging and Data Analysis
  • 03:06Results: Representative Cellular Bioluminescence Dynamics in Response to Chemical Exposure
  • 04:15Conclusion

Summary

Automatic Translation

哺乳动物细胞表达生物发光细菌基因盒(<em>勒克斯</em>),自主产生光。化学品接触后产生的生物发光动力学已被证明,以反映细胞生长和代谢​​的治疗效果,使这些细胞的一种廉价的,连续的,实时的毒性筛选的工具,可以很容易地适应高通量自动化。

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