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Imaging inlc Sekretion at undersøge Cellular Infektion med bakteriepatogen<em> Listeria monocytogenes</em
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Immunology and Infection
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JoVE Journal Immunology and Infection
Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Imaging inlc Sekretion at undersøge Cellular Infektion med bakteriepatogen<em> Listeria monocytogenes</em

DOI:
10.3791/51043-v

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14:05 min

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September 19, 2013

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, , , , , ,
1Unité des Interactions Bactéries Cellules,Pasteur Institute, 2INSERM U604, 3Institut National de la Recherche Agronomique (INRA), USC2020, 4Institute of Biochemistry,ETH Zürich, 5Focal Area Infection Biology, Biozentrum,University of Basel

Chapters

  • 00:05Title
  • 01:39Reverse siRNA Cell Transfection
  • 04:14Cellular Infection
  • 06:57Staining for InlC Protein
  • 09:19Results: Cell Infection by L. monocytogenes is Detected by Fluorescent Labeling of Cytoplasmic InlC
  • 13:27Conclusion

Summary

Automatic Translation

Automatic Translation

Listeria monocytogenes er en grampositiv bakteriel patogen, der ofte anvendes som en vigtig model til undersøgelse af intracellulære parasitter. Imaging sent L. monocytogenes infektion faser i forbindelse med små interfererende RNA skærme giver mulighed for global undersøgelse af cellulære veje er nødvendig for bakteriel infektion af target værtsceller.

Tags

Listeria MonocytogenesBacterial PathogenIntracellular ParasitismCellular Immune ResponseInlCFluorescent LabelingMicroscopyHigh-throughput SiRNA ScreeningCellular Signaling Pathways

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